The obtained CD-BPTI and CD-lysozyme conjugates were characterized by mass spectrometry. In that paper we presented the possibility of creating CD-protein conjugates, and the best conditions for carrying out this reaction have been determined. We have previously reported the usefulness of mono-6- O-formyl-β-CD in the modification of proteins in thermal reactions in solid state. Also, experiments on albumin, fibrinogen-methotrexate and lysozyme glycation show that careful choice of temperature and reaction time ensures the retention of the biological activity of the proteins. Then, this approach was further developed by Boratyński and Roy as a method for protein glycation and the synthesis of biologically active neoglycoconjugates. The solid state, thermal reaction between proteins and reducing sugar was first described by Lea. These conjugates are promising for delivery of anticancer drugs. Supramolecular and covalent CD-protein conjugates has been of great interest in the field of protein engineering. Ĭyclodextrins, due to their possibility to form host–guest complexes with many drugs, can be used to modify the structure of proteins. Furthermore, by affecting the intermolecular interactions of proteins CDs can interfere with their oligomerization and aggregation processes causing a stabilizing effect. ĬDs based rotaxans and polyrotaxanes have been employed as delivery carriers. For this reason CD-based nanosponges could be used as stable delivery systems and innovative drug carries for therapeutic purposes. ![]() They can be used for protecting easily degradable molecules and also could be used to improve the solubility of poorly soluble drugs. Also cyclodextrin-based nanosponges can form complexes with many lipophilic and hydrophilic drugs. Additionally, cationic β-CD polymer derived nanoparticles have been found to be efficient non-viral delivery vectors for siRNA in humans. In vivo therapeutic efficacy has been reported for nanoparticles assembled from camptothecin conjugated β-CD polymers. One example of such carriers is diamide linked γ-cyclodextrin (γ-CD) dimers that have been described as molecular-scale delivery capsules for the anticancer agent curcumin. Given their biocompatibility, CDs have been used as host units for the synthesis of host–guest delivery carriers. Recently, these host–guest interactions have been adopted to assemble polymer nanoparticles for drug and gene delivery. Due to these major features, CDs can be utilized in different areas of medicinal chemistry. ![]() The characteristic properties of the substance, such as solubility, chemical reactivity, or spectral property, are changed after guest compound is encapsulated. Accordingly, CDs have an ability to encapsulate and solubilize hydrophobic guest species in water through host–guest complexation. Naturally occurring CDs are composed of six, seven or eight d-glucopyranoside units in a toroidal structure, possessing a hydrophobic interior and hydrophilic exterior. ![]() In addition, the paper presents the potential of protein-cyclodextrin conjugates to construct innovative bioactive molecules for biological and medical applications.Ĭyclodextrins (CDs) are cyclic oligosaccharides with significant application in the pharmaceutical, food and cosmetic industries. This report demonstrates the great potential of cyclodextrin as a modifying unit that can be used to modulate the properties of therapeutic proteins, additionally giving such conjugates the possibility to transport many therapeutic substances in the form of inclusion complexes. The presented conjugates were biologically active and covalently bound β-cyclodextrin preserved the ability to form inclusion complexes with the model compound. The structure of the obtained conjugates was investigated via liquid chromatography-mass spectrometry, dynamic light scattering and circular dichroism analysis. The paper reports the solid state, thermal method for protein coupling with β-cyclodextrin and the physicochemical and biological properties of the obtained conjugates. ![]() Recently a great interest in the field of protein engineering and the design of innovative drug delivery systems employing specific ligands such as cyclodextrins is observed.
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